Project 1 - Physical modeling and dynamics of tumor cell migration mechanics
Aim 1: Stratify low-passage Glioblastoma and Pancreatic Ductal Adenocarcinoma patient-derived cancer cells using CMS1.0. We will parameterize the CMS1.0 using a collection of low-passage, patient-derived GBM and PDA cells migrating on compliant 2D surfaces of varying extracellular matrix composition.
Aim 2: Mechanically parameterize tumor-associated immune cells in GBM and PDA.
Aim 3: Elucidate the intracellular mechanism of novel genetic drivers in Medulloblastoma (MB) and PDA via a model-driven reverse genetics approach.
Download V 1.0 of the Cell Migration Simulator here
Project 2 - Cell Migration in Mechanically Complex Environments
Aim 1: Dissect the relative influence of contact guidance and durotaxis in complex 3D microenvironments during disease progression.
Aim 2: Expand the scope of the cell migration simulator by incorporating tissue architecture and mechanics.
Aim 3: Test the hypothesis that physical cues in the cellular microenvironment drive communication between tumor cell populations and regulate immune cell infiltration and function.